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Cells
Volume 13
Issue 12
10.3390/cells13121008
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Open AccessArticle
by Tamara Meijer SciProfilesScilitPreprints.orgGoogle Scholar Daniel da Costa Pereira SciProfilesScilitPreprints.orgGoogle Scholar Olivia C. Klatt SciProfilesScilitPreprints.orgGoogle Scholar Joanne Buitenhuis SciProfilesScilitPreprints.orgGoogle Scholar Paul Jennings SciProfilesScilitPreprints.orgGoogle Scholar Anja Wilmes SciProfilesScilitPreprints.orgGoogle Scholar
Tamara Meijer
, Daniel da Costa Pereira, Olivia C. KlattJoanne BuitenhuisPaul JenningsAnja Wilmes
1
Department of Chemistry and Pharmaceutical Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands
2
Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit Amsterdam, De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands
*
Author to whom correspondence should be addressed.
Cells 2024, 13(12), 1008; https://doi.org/10.3390/cells13121008 (registeringDOI)
Submission received: 24 May 2024/Accepted: 5 June 2024/Published: 9 June 2024
(This article belongs to the Special Issue Advancing Human Cell Culture Towards More Physiological, Differentiated Tissues-Series II)
Abstract
The polarised expression of specific transporters in proximal tubular epithelial cells is important for the renal clearance of many endogenous and exogenous compounds. Thus, ideally, the in vitro tools utilised for predictions would have a similar expression of apical and basolateral xenobiotic transporters as in vivo. Here, we assessed the functionality of organic cation and anion transporters in proximal tubular-like cells (PTL) differentiated from human induced pluripotent stem cells (iPSC), primary human proximal tubular epithelial cells (PTEC), and telomerase-immortalised human renal proximal tubular epithelial cells (RPTEC/TERT1). Organic cation and anion transport were studied using the fluorescent substrates 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (ASP) and 6-carboxyfluorescein (6-CF), respectively. The level and rate of intracellular ASP accumulation in PTL following basolateral application were slightly lower but within a 3-fold range compared to primary PTEC and RPTEC/TERT1 cells. The basolateral uptake of ASP and its subsequent apical efflux could be inhibited by basolateral exposure to quinidine in all models. Of the three models, only PTL showed a modest preferential basolateral-to-apical 6-CF transfer. These results show that organic cation transport could be demonstrated in all three models, but more research is needed to improve and optimise organic anion transporter expression and functionality.
Keywords: renal proximal tubule; human induced pluripotent stem cells; organic cation transport; organic anion transport
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MDPI and ACS Style
Meijer, T.; da Costa Pereira, D.; Klatt, O.C.; Buitenhuis, J.; Jennings, P.; Wilmes, A.Characterization of Organic Anion and Cation Transport in Three Human Renal Proximal Tubular Epithelial Models. Cells 2024, 13, 1008.https://doi.org/10.3390/cells13121008
AMA Style
Meijer T, da Costa Pereira D, Klatt OC, Buitenhuis J, Jennings P, Wilmes A.Characterization of Organic Anion and Cation Transport in Three Human Renal Proximal Tubular Epithelial Models. Cells. 2024; 13(12):1008.https://doi.org/10.3390/cells13121008
Chicago/Turabian Style
Meijer, Tamara, Daniel da Costa Pereira, Olivia C. Klatt, Joanne Buitenhuis, Paul Jennings, and Anja Wilmes.2024. "Characterization of Organic Anion and Cation Transport in Three Human Renal Proximal Tubular Epithelial Models" Cells 13, no. 12: 1008.https://doi.org/10.3390/cells13121008
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.
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MDPI and ACS Style
Meijer, T.; da Costa Pereira, D.; Klatt, O.C.; Buitenhuis, J.; Jennings, P.; Wilmes, A.Characterization of Organic Anion and Cation Transport in Three Human Renal Proximal Tubular Epithelial Models. Cells 2024, 13, 1008.https://doi.org/10.3390/cells13121008
AMA Style
Meijer T, da Costa Pereira D, Klatt OC, Buitenhuis J, Jennings P, Wilmes A.Characterization of Organic Anion and Cation Transport in Three Human Renal Proximal Tubular Epithelial Models. Cells. 2024; 13(12):1008.https://doi.org/10.3390/cells13121008
Chicago/Turabian Style
Meijer, Tamara, Daniel da Costa Pereira, Olivia C. Klatt, Joanne Buitenhuis, Paul Jennings, and Anja Wilmes.2024. "Characterization of Organic Anion and Cation Transport in Three Human Renal Proximal Tubular Epithelial Models" Cells 13, no. 12: 1008.https://doi.org/10.3390/cells13121008
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.
Cells,EISSN 2073-4409,Published by MDPI
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